Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 411, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.125106
Keywords
Nanozymes; Gold nanoclusters; Thiamine; Total antioxidant capacity; Fluorescence
Categories
Funding
- National Natural Science Foundation of China [21705056, 21902061, 21902062, 21904048]
- Young Taishan Scholars Program [tsqn201812080]
- Youth Innovative Talents Recruitment and Cultivation Program of Shandong Higher Education
- Natural Science Foundation of Shandong Province [ZR2019YQ10, ZR2018BB057, ZR2020QB033]
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A fluorescent assay for total antioxidant capacity detection has been developed using the light-responsive oxidase-like activity of BSA-AuNCs. This method enables the rapid oxidation of TH under neutral conditions and has been successfully utilized for detecting antioxidants and vitamin C tablets.
A fluorescent assay for total antioxidant capacity (TAC) detection based on the light-responsive oxidase-like activity of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) has been developed. Thiamine (TH) as the peroxidase substrate usually works at alkaline conditions and thus limits its practical applications. Here, by utilization the light-responsive oxidase-like activity of BSA-AuNCs, TH is oxidized to fluorescent thiochrome under neutral condition in two minutes due to the single oxygen generated by BSA-AuNCs upon light irradiation. After the introduction of antioxidants into the BSA-AuNCs-TH system, the formation of thiochrome is inhibited resulting in the fluorescence decrease. On the basis of the above facts, BSA-AuNCs-TH-based assay has been fabricated and applied successfully to detect antioxidants and the TAC of vitamin C tablets as well as some commercial fruit juice with satisfied results. This work may provide novel insights into developing lightresponsive nanozymes-based fluorescent assays.
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