Fatigue of anammox consortia under long-term 1,4-dioxane exposure and recovery potential: N-kinetics and microbial dynamics

Long-term exposure of anammox process to 1,4-dioxane was investigated using periodic anammox baffled reactor (PABR) under different 1,4-dioxane concentrations. The results generally indicated that PABR (composed of 4 compartments) has robust resistance to 10 mg-dioxane/L. The 1st compartment acted as a shield to protect subsequent compartments from 1,4-dioxane toxicity through secretion of high extracellular polymeric substance (EPS) of 152.9 mg/gVSS at 10 mg-dioxane/L. However, increasing 1,4-dioxane to 50 mg/L significantly inhibited anammox bacteria; e.g., similar to 93% of total nitrogen removal was lost within 14 days. The inhibition of anammox process at this dosage was most likely due to bacterial cell lysis, resulting in the decrease of EPS secretion and specific anammox activity (SAA) to 105.9 mg/gVSS and 0.04 mg N/gVSS/h, respectively, in the 1st compartment. However, anammox bacteria were successfully self-recovered within 41 days after the cease of 1,4-dioxane exposure. The identification of microbial compositions further emphasized the negative impacts of 1,4-dioxane on abundance of C. Brocadia among samples. Furthermore, the development of genus Planococcus in the 1st compartment, where removal of 1,4-dioxane was consistently observed, highlights its potential role as anoxic 1,4-dioxane degrader. Overall, long-term exposure to 1,4-dioxane should be controlled not exceeding 10 mg/L for a successful application.

Automated summary

Long-term exposure to 1,4-dioxane should not exceed 10 mg/L for successful application, as higher concentrations can significantly inhibit anammox bacteria. The first compartment of the PABR acts as a shield against the toxicity of 1,4-dioxane through high EPS secretion, but prolonged exposure at higher concentrations can lead to bacterial lysis. Despite initial inhibition, anammox bacteria were able to recover after cesation of 1,4-dioxane exposure.