Simultaneous removal of calcium, fluoride, nickel, and nitrate using microbial induced calcium precipitation in a biological immobilization reactor

In this research, an immobilized biofilm reactor was established for the simultaneous removal of calcium (Ca2+), fluoride (F-), nickel (Ni2+), and nitrate (NO3- -N) by microbial induced calcium precipitation (MICP). The operating parameters of the reactor, hydraulic retention time (HRT: 4, 8, and 12 h), influent Ca2+ concentration (36.0, 108.0, and 180.0 mg L-1), and influent Ni2+ concentration (0.0, 3.0, and 6.0 mg L-1) were discussed. Under the HRT of 12 h, influent Ca2+ concentration of 180.0 mg L-1, and influent Ni2+ concentration of 3.0 mg L-1, the removal ratios of Ca2+, F-, Ni2+, and NO3- -N reached 45.31%, 79.55%, 85.11%, and 55.29%, respectively, which was the reactor stable operation performance. The SEM revealed the morphology of calciumprecipitated bio-crystals. XPS showed the Ca2+ and Ni2+ precipitate components and XRD further revealed the formation of CaCO3, Ca-5(PO4)(3)OH, and NiCO3 precipitation. Nitrogen (N-2) was the main gas produced in the reactor. Fluorescence spectroscopy manifested that extracellular polymers played an important role in the organism nucleation. High-throughput sequencing exhibited that Acinetobacter sp. H-12 was the dominant bacterial group. This study provided a new insight for simultaneous remediation of Ca2+, F-, Ni2+, and NO3- -N in water bodies.

Automated summary

This study established an immobilized biofilm reactor for the simultaneous removal of calcium, fluoride, nickel, and nitrate by microbial induced calcium precipitation. By analyzing reactor operating parameters and component precipitates, it demonstrated effective removal of targeted substances from water bodies. High-throughput sequencing identified Acinetobacter sp. H-12 as the dominant bacterial group involved in the process.