Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 414, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.125582
Keywords
Dose metric; Curvature; Silver nanoparticle; Human serum albumin; Cytotoxicity
Categories
Funding
- National Natural Science Foundation of China [21677159, 21806180, 22076065]
- Science and Technology Support Plan for Youth Innovation of Colleges in Shandong Province [DC2000000961]
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The major binding protein on silver nanoparticles (SNPs) in blood was identified as human serum albumin (HSA), and the protein corona significantly alleviated the toxicity of SNPs, with more pronounced effects on larger particles. This study highlights the importance of protein interactions and dose metrics in understanding nanotoxicity and designing safe nanomaterials.
With the potential biomedical applications of nanomaterials such as silver nanoparticles (SNPs), nanotoxicity concerns are growing, and the importance of NP and protein interactions is far from being addressed enough. Here, we identified the major binding protein on SNPs in blood as human serum albumin (HSA) using polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry. By comparing with the previous methods, we emphasized surface area concentration as a new dose metric to address the importance of NP curvature. SNPs interacted with cysteine and cystine, disrupting the secondary structure and conformation of HSA, and this tendency became stronger on small SNPs than large ones. The protein corona significantly alleviated the toxicity and decreased SNPs' internalization in a particle size-dependent manner, where more significant inhibition effects occurred on larger particles at the same area concentration. These findings may shed light on nanotoxicity and also the design of safe nanomaterials by a comprehensive preconsideration of the metrological method.
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