The molecular-level understanding of the uptake of PFOS and its alternatives (6:2 Cl-PFESA and OBS) into phospholipid bilayers

Bioaccumulation of perfluoroalkyl and polyfluoroalkyl substances (PFASs) is an important indicator of their hazard. Partitioning to membrane phospholipids is one of the pathways for their bioaccumulation. However, the molecular mechanism on PFASs uptake into membrane phospholipids is not yet to be fully understood. In this work, we used molecular dynamics (MD) simulations to study the uptake processes of PFOS and its alternatives (6:2 Cl-PFESA and OBS) into DPPC bilayers, and to evaluate their interaction with DPPC bilayers and their effect on properties of DPPC bilayers. The result of free energy changes shows that a barrier of 2-3 kcal mol-1 exists when these adsorbed PFASs on the surface are absorbed into DPPC bilayers. After incorporating into DPPC bilayers, three DPPC molecules interact with and thus stabilize a PFOS (or 6:2 Cl-PFESA or OBS) molecule. And another role of the three DPPC molecules is to shield these PFASs from exposure to water environment. These PFASs have the similar condensing effect on the model membrane. The molecular-level study is beneficial for understanding the bioaccumulation and toxicity of PFOS and its alternatives.

Automated summary

Through molecular dynamics simulations, the study investigated the uptake processes of PFOS and its alternatives into DPPC bilayers, revealing a barrier when the PFASs are absorbed into the bilayers. The interaction between three DPPC molecules and a PFAS molecule stabilizes the PFAS molecule while shielding it from exposure to water environment. The study sheds light on the bioaccumulation and toxicity of PFOS and its alternatives.