Journal
FOOD MICROBIOLOGY
Volume 55, Issue -, Pages 123-127Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2015.11.002
Keywords
Acinetobacter spp. detection; Enrichment; Selective growth; Food matrix
Funding
- National Funds from FCT - Fundacao para a Ciencia e a Tecnologia [PEst-OE/EQB/LA0016/2013]
- project MAISEGURO - the European Community Fund FEDER through ON. 2 - O Novo Norte - North Portugal Regional Operational Programme
- [SFRH/BD/72951/2010 FCT]
- [SFRH/BPD/35392/2007 FCT]
- [SFRH/BPD/72617/2010 FCT]
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Relatively little is known about the role of foods in the chain of transmission of acinetobacters and the occurrence of different Acinetobacter spp. in foods. Currently, there is no standard procedure to recover acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of acinetobacters. This study aimed to assess whether enrichment in Dijkshoorn enrichment medium followed by plating in CHROMagar (TM) Acinetobacter medium is a useful method for the isolation of Acinetobacter spp. from foods. Recovery of six Acinetobacter species from food spiked with these organisms was compared for two selective enrichment media (Baumann's enrichment and Dijkshoorn's enrichment). Significantly (p < 0.01) higher cell counts were obtained in Dijkshoorn's enrichment. Next, the Dijkshoorn's enrichment followed by direct plating on CHROMagar (TM) Acinetobacter was applied to detect Acinetobacter spp. in different foods. Fourteen different presumptive acinetobacters were recovered and assumed to represent nine different strains on the basis of REP-PCR typing. Eight of these strains were identified by rpoB gene analysis as belonging to the species Acinetobacter johnsonii, Acinetobacter calcoaceticus, Acinetobacter guillouiae and Acinetobacter gandensis. It was not possible to identify the species level of one strain which may suggests that it represents a distinct species. (C) 2015 Elsevier Ltd. All rights reserved.
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