Improvement of chitosan production from Persian Gulf shrimp waste by response surface methodology

Chitosan is a water soluble polysaccharide that was extracted from the wastes of Persian Gulf shrimp. It has been a big challenge for the scientists to find different biological, mechanical, physical, enzymatic and microbial procedures to extract edible chitosan. In this study chitosan (isolated from waste of species of Indian white shrimp) with high functionality was produced using mild conditions (temperature (60, 80 and 100 degrees C), concentration of alkaline (30, 40 and 50%) and time (90, 195, 300 min) of reaction in chemical method and power of microwave (300, 600 and 900 W), concentration of alkaline (30, 40 and 50%) and irradiation time (20, 100, 180 S) in microwave method) and short duration which did not degrade the structure of chitosan. It was observed that the highest percent of chitosan preparation (19.47%), degree of deacetylation (89.34%) and molecular weight (806,931 Da) were achieved in microwave style at 50% NaOH solution, 720 W microwave power and 20 S reaction time. (C) 2016 Published by Elsevier Ltd.