4.4 Article

Biological Synthesis of PbS, As3S4, HgS, CdS Nanoparticles using Pseudomonas aeruginosa and their Structural, Morphological, Photoluminescence as well as Whole Cell Protein Profiling Studies

Journal

JOURNAL OF FLUORESCENCE
Volume 31, Issue 5, Pages 1445-1459

Publisher

SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-021-02769-2

Keywords

PbS; As3S4; HgS; CdS; Pseudomonas aeruginosa; Metal sulphide

Funding

  1. Conacyt [746, 299 058]
  2. Ciencias Fronteras Project [CF19-2 096 029]

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Metal sulfide nanoparticles were synthesized using Pseudomonas aeruginosa from textile effluent, showing optical, structural, and morphological characteristics. Analysis revealed functional groups on the bacteria surface aiding metal binding, with changes in protein concentration and banding pattern under heavy metal stress.
Y Metal sulfide nanoparticles are semi-conductors that possess many applications in optics, optoelectronics and magnetic devices. There are physical and chemical methods for their synthesis but such methods involve toxic precursors as well as many obnoxious by-products. Hence, biological synthesis of metal sulfide nanoparticles are efficient enough to transform toxic metals to non-toxic ones. Pseudomonas aeruginosa, isolated from textile effluent and tolerant of high levels of heavy metals, was used for the green synthesis of metal sulfide (HgS, As3S4, CdS and PbS) nanoparticles. The optical, structural and morphological nature of metal sulfide nanoparticles was also determined. FTIR (Fourier Transform Infra-red) analysis showed spectral changes when P. aeruginosa was grown in medium containing heavy metals viz. Hg, As, Pb and Cd indicating that there are functional groups viz. carboxyl, hydroxyl, phosphate, amino and amide, that exists on the surface of the bacteria, thus facilitating binding of metals on its surface. The bacterial samples which were treated with different metals at different concentrations, were subjected to whole cell protein analysis using SDS-PAGE (Sodium dodecyl Sulphate- Polyacrylamide gel electrophoresis) and protein profiling. The total protein estimation revealed that there was an increase in the protein concentration in the presence of heavy metals and a significant change in the banding pattern was observed which showed induction of a set of proteins under heavy metal stress especially mercury.

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