Silicon combined with foliar melatonin for reducing the absorption and translocation of Cd and As by Oryza sativa L. in two contaminated soils

Potentially toxic elements (PTE) toxicity has serious effects for human health. Si has been tested to investigate their ability to mitigate Cd and As contamination of rice. In this study, the combined effect of Si and melatonin (MT) on Cd and As uptake and transport in rice plants is tested in two contaminated soils via controlled pot experiments. Results showed that a combined Si and MT treatment (Si + MT) was more effective at reducing Cd and As uptake and transport than Si alone. The treatment had the strongest effect on Cd concentrations in rice grains from high-polluted soil (HP) when treated at the flowering stage (81.8% reduction) and from low-polluted soil (LP) at the tillering stage (TS, 64.9%). The greatest reduction of grain As was found when treated at TS in both soils, by 58.2% and 39.2% in HP and LP soil, respectively. The significant upregulation of CAT, SOD, and POD activities, and downregulation of MDA by Si + MT was more effective than that of Si alone; Si + MT significantly decreased expressions of Nramp1, HMA2, and IRT2 in roots in both soils, and also Nramp5, HMA3, and IRT1 in LP soil, which might result in Si+MT effect on Cd and As accumulation. However, Si + MT had little effect on the amino acid content of grains compared to Si alone. Overall, the combination of Si and MT was substantially more effective at reducing Cd and As uptake and transport than Si alone, especially in HP soil. This effect might result from the regulation of antioxidant potential and gene expression relating Cd uptake and transport.

Automated summary

The combination of silicon (Si) and melatonin (MT) was found to be more effective in reducing the uptake and transport of cadmium (Cd) and arsenic (As) in rice plants compared to Si alone. The Si+MT treatment significantly decreased Cd and As concentrations in rice grains, with the greatest impact seen in high-polluted soil (HP) for Cd reduction and in low-polluted soil (LP) for As reduction. This effect may be attributed to the regulation of antioxidant potential and gene expression related to Cd uptake and transport.