Journal
FOOD CONTROL
Volume 60, Issue -, Pages 302-308Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2015.07.040
Keywords
Enterotoxin-producing staphylococci; qPCR; Meat products
Categories
Funding
- project Camisenusa, Consolider Ingenio 2010 and GR10162 of the Gobierno de Extremadura and FEDER [CSD2007-00016]
- University of Extremadura
- Gobierno de Extremadura
- MICINN
- FEDER
- FSE
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A SYBR Green-based real-time PCR (qPCR) procedure for the rapid and specific detection of enterotoxin-producing Staphylococcus spp. in meat products has been developed. For this, a specific primer pair based on conserved regions of enterotoxin genes was designed for detecting most of the described staphylococcal enterotoxins. No cross-reactivity with other microorganisms or non-enterotoxin-producing Staphylococcus spp. was detected. The detection limits of the assay were about 2-40 cfu/g for artificially contaminated meat products after 8 h enrichment period at 30 degrees C. Total time for assay completion was approximately 12 h. Thus, the qPCR method offers a useful, rapid and efficient tool for screening enterotoxin-producing Staphylococci in meat products. This tool could be also used for monitoring these foodborne pathogens in food safety preventive programs. (c) 2015 Elsevier Ltd. All rights reserved.
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