The Effect of Mechanical Vibration on Osteogenesis of Periodontal Ligament Stem Cells

Introduction: Appropriate occlusal forces can prevent ankylosis after tooth replantation or transplantation. However, the proper occlusal forces on periodontal ligament (PDL) healing have not yet been defined due to insufficient in vitro studies and uncertain in vitro models. Herein, we presented a mechanical vibration device as an in vitro model to determine such favorable occlusal forces. Methods: Human periodontal ligament stem cells (hPDLSCs) were exposed to mechanical vibration force with 4 frequencies (30, 90, 150, and 210 rpm). Cell viability and the expression of osteogenic differentiation-related genes and proteins were tested in vitro. The calvarial transplantation experiment was performed to assess the bone formation ability of 150 rpm mechanical vibration stimulation (MVS). Results: MVS at 150 and 210 rpm significantly reduced cell viability in the early stages. The 150-rpm MVS decreased osteogenic marker expression at the early time point (3 days) but had no harmful effects at the late time point (14 days). Furthermore, hPDLSC cell sheets treated with 150-rpm MVS had potential to decrease bone formation in rat calvarial defects serendipitously and facilitated functional PDL-like tissue formation. Conclusions: We found that MVS at a frequency of 150 rpm could provide a strategy for a transient reduction in the osteogenic potential of hPDLSCs and promote PDL-like tissue formation. Thus, 150-rpm MVS could be used as a controllable proper occlusal force to prevent ankylosis and promote PDL healing after tooth replantation or transplantation. (J Endod 2021;47:1767-1774.) SIGNIFICANCE One hundred fifty-rpm MVS had a decreased osteogenic potential and contributed to PDL formation, which suggests that 150-rpm MVS might provide a therapeutic value to reduce ankylosis and improve PDL healing after tooth replantation and transplantation.

Automated summary

The study showed that mechanical vibration at 150 rpm could transiently reduce the osteogenic potential of human periodontal ligament stem cells and promote the formation of PDL-like tissue. This suggests that 150-rpm MVS may be a therapeutic approach to prevent ankylosis and improve PDL healing after tooth replantation and transplantation.