Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 895, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2021.115463
Keywords
Magnetic beads; Screen-printed electrode; Parathyroid hormone
Categories
Funding
- Ministry of Science and Technology [MOST 1082221E182039]
- Chang Gung University [BMRP 758]
- Chang Gung Memorial Hospital [2 J0161, 2 J0162, 2H0072, 2H0073]
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A highly sensitive magneto immunosensor for detecting parathyroid hormone (PTH) was successfully developed using MBs modified by APBA and a disposable electrode, allowing for rapid point-of-care testing for PTH. The sensor exhibited a linear detection range and low limits of detection by both voltammetry and impedance spectroscopy, showcasing its potential for practical diagnostic applications.
Using magnetic beads (MBs) in electrochemical immunosensors has been shown to improve their sensitivity, reduce analysis time, and minimize reaction volume. The antigen-antibody interaction can be improved with the correct orientation of the antibody using aminophenyl boronic acid (APBA) as the immobilized ligand. In this work, a highly sensitive magneto immunosensor to detect parathyroid hormone (PTH) was reported. The analysis was based on the immobilization of anti-PTH antibodies onto MBs followed by immunocapture, voltammetric and impedimetric assay. We introduce a facile two-step method to detect PTH by using MBs modified by APBA and antibody-peroxidase complex. Immunocapture of PTH by the antibodies takes place on the modified MBs, and the immunocomplexes are separated by a magnet on a screen-printed electrode. Electrochemical detection was performed by measuring the catalytic current and impedance. The APBA coating, antibody-peroxidase ratio, MB characterization, and electrochemical parameters were systematically investigated in this study. The PTH immunosensor exhibited a linear range between 10 and 300 pg mL-1, with a limit of detection of 11.56 pg mL-1 by voltammetry and 49.30 pg mL-1 by impedance spectroscopy. A magneto immunosensor was successfully prepared by using modified MBs and a disposable electrode, which can be applied to analyze PTH quickly as a point of care device.
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