Amorphous carbon nitride microband integrated in a microfluidic device for DNA biosensors applications

This study presents the use of new kind of carbon electrode materials as ultramicroelectrodes (UMEs) in the field of electrochemical DNA biosensors which has already been proven to be effective in protocols to DNA sequences hybridization. In contrast to other carbon materials such as diamond like carbon, that are difficult to integrate in microfluidic devices due to their high temperature deposition, amorphous carbon nitride (aCNx) is easily synthesized at room temperature on various materials using sputtering techniques. Here, we report a-CNx use as microband electrodes in Glass/PDMS microfluidic devices. a-CNx electrodes were activated and then biofuntionalized by covalent grafting of a DNA probe as self-assembled monolayer (SAM) with a view to future development of a detection platform targeting circulating DNA or RNA sequences in microfluidic channels.

Automated summary

This study demonstrates the effectiveness of new carbon electrode materials, particularly amorphous carbon nitride, in electrochemical DNA biosensors. These materials can be easily synthesized at room temperature and integrated into microfluidic devices, showing potential for future development targeting circulating DNA or RNA sequences in microfluidic channels.