Journal
JOURNAL OF ECONOMIC ENTOMOLOGY
Volume 114, Issue 6, Pages 2610-2614Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/jee/toab168
Keywords
pest management; molecular diagnostic; fall armyworm
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Funding
- Department of Primary Industries and Regional Development (DPIRD) Building Grains Project
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Fall armyworm is an economically important pest worldwide and has been identified in Australia. A sensitive, specific, and in-field capable loop-mediated isothermal amplification (LAMP) assay has been developed for rapid and accurate detection of S. frugiperda larvae, which can greatly assist with timely management decisions.
Fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae), is an economically important pest worldwide and has recently been identified in Australia. Morphological identification of S. frugiperda at early larval stages can be difficult often requiring expert microscopy analysis. Rapid and accurate in-field diagnosis is vital for management decision support and there are no tools currently available for this purpose. In this study, a sensitive, specific, and in-field capable loop-mediated isothermal amplification (LAMP) assay was developed to detect S. frugiperda larvae. A primer set based on a highly conserved region of the S. frugiperda cytochrome oxidase subunit 1 (COX1) gene provided detection within 30 min from both total DNA and crude extractions. The crude extraction technique of crushing 10 mg of S. frugiperda material in 50 mu l ddH(2)O and further diluting the homogenate in ddHp is rapid, simple, and does not require heat blocks, centrifuges, or special buffers increasing its utility as a field-based technique. The primer set detected as little as 24 pg of S. frugiperda DNA and did not cross-react with any other of the lepidopteran species tested that are easily confused with S. frugiperda in Australia. Therefore, this assay could be used in-field to correctly identify the presence of S. frugiperda and thereby greatly assist with timely management decisions.
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