Journal
JOURNAL OF DENTISTRY
Volume 109, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jdent.2021.103655
Keywords
Tailored amorphous multiporous (TAMP); Calcium silicate; Dentin-pulp; Dental pulp stem cells; Tissue regeneration; Regenerative endodontics; Bioactive materials
Categories
Funding
- NSF [IIP-1602057]
- National Institutes of Health [R01 DE019156]
- University of Tennessee Health Science Center
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The TAMP calcium silicate material showed good biocompatibility with human and swine DPSCs in vitro, and demonstrated positive outcomes for pulp regeneration in vivo, suggesting its potential application in dentin bridge regeneration after pulp exposure.
Introduction/Objective: The tailored amorphous multi-porous (TAMP) material fabrication technology has led to a new class of bioactive materials possessing versatile characteristics. It has not been tested for dental applications. Thus, we aimed to assess its biocompatibility and ability to regenerate dental mineral tissue. Methods: 30CaO-70SiO(2) model TAMP discs were fabricated by a sol-gel method followed by in vitro biocompatibility testing with isolated human or mini-swine dental pulp stem cells (DPSCs). TAMP scaffolds were tested in vivo as a pulp exposure (pin-point, 1 mm, 2 mm, and entire pulp chamber roof) capping material in the molar teeth of mini-swine. Results: The in vitro assays showed that DPSCs attached well onto the TAMP discs with comparable viability to those attached to culture plates. Pulp capping tests on mini-swine showed that after 4.5 months TAMP material was still present at the capping site, and mineral tissue (dentin bridge) had formed in all sizes of pulp exposure underneath the TAMP material. Conclusions: TAMP calcium silicate is biocompatible with both human and swine DPSCs in vitro and with pulp in vivo, it may help regenerate the dentin bridge after pulp exposure.
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