Journal
JOURNAL OF DAIRY SCIENCE
Volume 104, Issue 12, Pages 12207-12215Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2021-20471
Keywords
donkey colostrum; bovine colostrum; milk fat globule membrane proteins; proteomics
Funding
- National Key R&D Program of China [2018YFC1604302]
- Twelfth Five Year National Science and Technology Plan Project of China [2013BAD18B03]
- China Scholarship Council [202008210391]
- Shenyang Technological Innovation Project (China) [Y17-0-028]
- LiaoNing Revitalization Talents Project (China) [XLYC1902083]
Ask authors/readers for more resources
This study used quantitative proteomics to analyze milk fat globule membrane (MFGM) proteins in donkey colostrum (DC) and bovine colostrum (BC). A total of 179 (DC) and 195 (BC) MFGM proteins were identified, with 71 shared, 108 DC-specific, and 124 BC-specific proteins. Gene ontology analysis showed enrichment in cellular components and metabolic pathways, with 51 differentially expressed MFGM proteins identified.
This study was designed to provide novel insights into milk fat globule membrane (MFGM) proteins in donkey colostrum (DC) and bovine colostrum (BC) using quantitative proteomics. In total, 179 (DC) and 195 (BC) MFGM proteins were characterized, including 71 shared, 108 DC-specific, and 124 BC-specific proteins. Fifty-one shared proteins were selected as differentially expressed MFGM proteins, including 21 upregulated and 30 downregulated proteins in DC. Gene ontology analysis showed that these proteins were mainly enriched in cellular components, including the extracellular exosome, extracellular space, and plasma membrane. Additionally, they were further involved in metabolic pathways, including cholesterol metabolism, the peroxisome proliferator-activated receptor signaling pathway, and purine metabolism. Furthermore, several key protein factors with high connectivity were identified via protein-protein interaction analysis. These results provide more comprehensive knowledge of differences in the biological properties of MFGM proteins in DC and BC as well as pave the way for future studies of the nutritional and functional requirements of these important ingredients toward the development of dairy products based on multiple milk sources.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available