Journal
FOOD CHEMISTRY
Volume 190, Issue -, Pages 331-337Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.05.120
Keywords
alpha-Amylase inhibitor; Antioxidative; Bioactive peptides; Pinto bean
Funding
- ERGS Grant [203/CAATS/6730103]
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Antioxidant and a-amylase inhibitor peptides were successfully extracted from Pinto bean protein isolate (PBPI) using Protamex. A factorial design experiment was conducted and the effects of extraction time, pH and temperature were studied. pH 7.5, extraction time of 1 h, S/E ratio of 10 (w/w) and temperature of 50 degrees C gave the highest antioxidant activities (i.e., ABTS scavenging activity (53.3%) and FRAP value (3.71 mM)), whereas pH 6.5 with the same extraction time, S/E ratio and temperature, gave the highest a-amylase inhibitory activity (57.5%). It was then fractioned using membrane ultrafiltration with molecular weight cutoffs of 100, 50, 30, 10 and 3 kDa. Peptide fraction <3 kDa, which exhibited the highest antioxidant activities (i.e., ABTS (42.2%) and FRAP (0.81 mM)) and a-amylase inhibitory activity (62.1%), was then subjected to LCMS and MS/MS analyses. Six sequences were identified for antioxidant peptides, whereas seven peptides for a-amylase inhibitor. (C) 2015 Elsevier Ltd. All rights reserved.
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