Journal
FOOD CHEMISTRY
Volume 192, Issue -, Pages 799-804Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.07.080
Keywords
OTA; Cocoa beans; Food safety; DPV; Competition based aptasensor
Funding
- European project COCOtA: MINISTERE DE L'AGRICULTURE, DE L'AGROALIMENTAIRE ET DE LA FORET
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In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15-5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1-85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices. (C) 2015 Elsevier Ltd. All rights reserved.
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