Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 594, Issue -, Pages 409-423Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2021.03.053
Keywords
Vascular adhesion; Nanoparticles; Permeability; Microfluidic
Categories
Funding
- European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant [616695]
- European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant [754490]
- Ministry of Foreign Affairs and International Cooperation [PGR00796]
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The selective permeation of molecules and nanomedicines across the diseased vasculature can be effectively analyzed using a double-channel microfluidic device. Permeation enhancers were found to promote the perivascular accumulation of particles smaller than 200 nm, while having no effect on larger particles in the proposed on-chip microvasculature. Additionally, the microfluidic device offers insights into the role of particle deformability in vascular dynamics.
Hypothesis: The selective permeation of molecules and nanomedicines across the diseased vasculature dictates the success of a therapeutic intervention. Yet, in vitro assays cannot recapitulate relevant differences between the physiological and pathological microvasculature. Here, a double-channel microfluidic device was engineered to comprise vascular and extravascular compartments connected through a micropillar membrane with tunable permeability. Experiments: The vascular compartment was coated by endothelial cells to achieve permeability values ranging from -0.1 gm/sec, following a cyclic adenosine monophosphate (cAMP) pre-treatment (25 gg/ mL), up to -2 gm/sec, upon exposure to Mannitol, Lexiscan or in the absence of cells. Fluorescent microscopy was used to monitor the vascular behavior of 250 kDa Dextran molecules, 200 nm polystyrene nanoparticles (PB), and 1,000 x 400 nm discoidal polymeric nanoconstructs (DPN), under different permeability and flow conditions. Findings: In the proposed on-chip microvasculature, it was confirmed that permeation enhancers could favor the perivascular accumulation of -200 nm, in a dose and time dependent fashion, while have no effect on larger particles. Moreover, the microfluidic device was used to interrogate the role of particle deformability in vascular dynamics. In the presence of a continuous endothelium, soft DPN attached to the vasculature more avidly at sub-physiological flows (100 gm/sec) than rigid DPN, whose deposition was larger at higher flow rates (1 mm/sec). The proposed double-channel microfluidic device can be effi-ciently used to systematically analyze the vascular behavior of drug delivery systems to enhance their tissue specific accumulation. (c) 2021 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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