Journal
FOOD CHEMISTRY
Volume 208, Issue -, Pages 309-317Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.04.012
Keywords
Choerospondias axillaris; Nutraceuticals; Antioxidants; Cellular-based methods; Proanthocyanidins; Structure-activity
Funding
- National Natural Science Foundation of China [31460394]
- State Key Laboratory of Food Science and Technology Projects [SKLF-ZZA-201304]
- Jiangxi Science & Technology Pillar Program [20121BBF60039]
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An extract isolated from Choerospondias axillaris peels was separated into five fractions using size-exclusion chromatography. The structural composition and mean degree of polymerization (mDP) of these fractions were then characterized by acid-catalysis followed by HPLC analysis. The antioxidant activity of each fraction was determined using a combination of chemical-based methods (DPPH center dot, ABTS(center dot+) radical scavenging activity, ferric-reducing antioxidant power, and phosphomolybdate assay) and a cellular-based assay. All fractions tested were found to have high total phenolics contents and were rich in proanthocyanidins. The mDP of fractions (F1-F5) ranged from 1.92 to 9.25. When tested by the chemical-based assays, the antioxidant activity of the fractions did not depend on molecular weight of the phenolics. Conversely, when tested by the cellular-based assay the antioxidant activity actually decreased with increasing molecular weight of the proanthocyanidins. These experiments highlight the limitations of using chemical-based assays to establish the antioxidant activity of proanthocyanidins within biological systems. (C) 2016 Elsevier Ltd. All rights reserved.
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