Journal
FOOD CHEMISTRY
Volume 198, Issue -, Pages 12-19Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.11.036
Keywords
DDGS; Protein; Wheat; Distillery; Extraction; In-process samples; Amino acids
Funding
- Integrated Biorefining Research and Technology Club (IBTI) of the UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J019429/1, BB/J019380/1]
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005206, BB/J019429/1, BB/J019380/1] Funding Source: researchfish
- BBSRC [BB/J019429/1, BBS/E/C/00005206, BB/J019380/1] Funding Source: UKRI
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Wheat Distillers' Dried Grains with Solubles (DDGS) and in-process samples were used for protein extraction. Prolamins were the predominant protein components in the samples. The absence of extractable alpha- and gamma-gliadins in DDGS indicated protein aggregation during the drum drying processing stage. Prolamin extraction was performed using 70% (v/v) ethanol or alkaline-ethanol solution in the presence of reducing agent. DDGS extracts had relatively low protein contents (14-44.9%, w/w), regardless of the condition applied. The wet solids were the most suitable raw material for protein extraction, with recovery yields of similar to 55% (w/w) and protein content of similar to 58% (w/w) in 70% (v/v) ethanol. Protein extracts from wet solids were significantly rich in glutamic acid and proline. Mass balance calculations demonstrated the high carbohydrate content (similar to 50%, w/w) of solid residues. Overall, the feasibility of utilising in-process samples of DDGS for protein extraction with commercial potential was demonstrated. (C) 2015 Elsevier Ltd. All rights reserved.
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