Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 59, Issue 10, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00990-21
Keywords
African swine fever virus; luciferase immunoprecipitation system; magnetic beads; rapid; sensitivity
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Funding
- Department of Science and Technology of Hubei province, China [2019ABA08]
- National Key Research and Development Program of China from the Ministry of Science and Technology of China [2018YFC0840402]
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The study developed a rapid and easy to operate MB-LIPS assay for detecting ASFV antibodies in swine sera, showing high sensitivity and accuracy with results available within 30 minutes.
African swine fever (ASF) is a highly contagious viral disease of domestic pigs and wild boars. For disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (MB-LIPS) to detect ASF virus (ASFV) antibody. The MB-LIPS is based on magnetic beads modified with protein A/G and the recombinant fusion protein of ASFV p30 and luciferase, where p30 functioned as the recognition element and luciferase as the signal component. Incubation and washing could be finished automatically on a machine with magnetic rods. Under optimal conditions, the MB-LIPS showed 96.3% agreement to a commercial enzyme-linked immunosorbent assay (ELISA) kit for detecting ASFV antibody in swine sera. Analyzing serial dilutions of a swine serum sample showed that the MP-LIPS assay was 4 times more sensitive than the ELISA kit. The whole run of the MB-LIPS could be completed within 30 min. With its high sensitivity and simple operation, the MB-LIPS platform has great potential to be used for the detection of ASFV antibody and ASF control in small labs and farms.
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