APOE gene e4 allele (388C-526C) effects on serum lipids and risk of coronary artery disease in southern Chinese Hakka population

Objective To analyze the relationship of Apolipoprotein E (APOE) and solute carrier organic anion transporter family member 1B1 (SLCO1B1) gene polymorphisms with coronary artery disease (CAD). Methods 1,129 CAD patients and 1,014 non-CAD controls were included in the study, and relevant information and medical records were collected. The single-nucleotide polymorphisms (SNPs) were analyzed, including rs429358, rs7412 in APOE gene and rs2306283, rs4149056 in SLCO1B1 gene. Results The CAD patients' average age was 66.3 +/- 10.7 years, while 65.5 +/- 12.0 years in controls. The frequencies of APOE allele e3, e4, and e2 were 83.01%, 10.08%, and 6.91% respectively. There were statistically significant differences in genotype e3/e4 (chi(2) = 8.077, p = 0.005) in CAD patients compared with the controls. The SLCO1B1 genotype *1b/*1b and haplotype *1b showed the highest frequency in the study sample. Moreover, epsilon 4 carriers had significantly lower HDL-C, Apo-A1 levels than epsilon 3 carriers among CAD patients, while epsilon 2 carriers showed lower LDL-C, Apo-B level, and higher Apo-A1/Apo-B level than epsilon 3 and epsilon 4 carriers. In controls, epsilon 2 carriers showed lower LDL-C and Apo-B level, higher Apo-A1, and Apo-A1/Apo-B level than epsilon 4 carriers. Logistic regression analysis showed that high LDL-C and Apo-B level, low HDL-C level, smoking, and the epsilon 4 allele were risks for the presence of CAD. Conclusions APOE epsilon 4 allele may be associated with susceptibility to CAD in southern Chinese Hakka population. It indicated that the APOE SNPs rs429358 and rs7412 are associated with CAD, but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene.

Automated summary

The study found that the APOE epsilon 4 allele may be associated with susceptibility to CAD in the southern Chinese Hakka population. It indicated that the APOE SNPs rs429358 and rs7412 are associated with CAD, but not the SLCO1B1 gene SNPs rs2306283 and rs4149056.