Journal
FOOD CHEMISTRY
Volume 196, Issue -, Pages 610-618Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.09.101
Keywords
Poplar buds; Active constituents; P. italicum; Mode of action; Respiration
Funding
- National Science Foundation of China [31271969, 31471633]
- Natural Science Foundation of Hubei Province, China [2011CDB147]
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Antifungal components, from poplar buds active fraction (PBAF) against Penicillium italicum, the causal agent of blue mold in citrus fruits, were identified and possible action modes were investigated. Pinocembrin, chrysin and galangin were determined as active components in PBAF, using HPLC and HPLC-MS analysis. The antifungal activity is stable at temperatures ranging from 4 degrees C to 100 degrees C and pH levels ranging from 4 to 8. In the presence of PBAF, the hyphae become shriveled, wrinkled and the cell membrane became seriously disrupted. Further investigation on cell permeability, nucleic acid content and alkaline phosphatase suggest that the cell membrane might be the target. Mycelial oxygen consumption and the respiration-related enzymatic activity of succinate dehydrogenase, malate dehydrogenase and ATPase were all inhibited by PBAF. We propose that PBAF is a potentially useful alternative for blue mold control and may act against P. italicum by interfering with respiration and disrupting the cell membrane. (C) 2015 Elsevier Ltd. All rights reserved.
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