Journal
FOOD CHEMISTRY
Volume 209, Issue -, Pages 279-285Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.04.083
Keywords
3-Methyl-quinoxaline-2-carboxylic acid; Hapten; Monoclonal antibody; ELISA
Funding
- Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture, PRC [201303085]
- Natural Science Foundation of Shandong Province of China [ZR2015BQ015]
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Two novel immunizing haptens of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) were synthesized and conjugated with cationized bovine serum albumin. Female BALB/c mice were immunized with above conjugates, splenocytes were fused with Sp2/0 cells to produce monoclonal antibody. Compared with previous studies, antibodies raised in this work showed higher sensitivity. Meantime, a novel heterologous coating hapten was also prepared. The indirect competitive enzyme-linked immunosorbent assay (icELISA) based on the optimum condition showed an IC50 of 3.1 mu g/kg (ppb), and the linear range of 0.46-10.5 ppb for MQCA. The limit of detect (LOD) of MQCA in swine muscle, swine liver and chicken was 0.32, 0.54, and 0.28 ppb, respectively. The LOD of this assay can satisfy the minimum required performance levels (4 ppb) for MQCA. These results indicated that the proposed ELISA, with high sensitivity and specificity, as well as good reproducibility and accuracy, is suitable for determination of MQCA residues in food samples. (C) 2016 Elsevier Ltd. All rights reserved.
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