Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1648, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2021.462168
Keywords
Solid-phase extraction; HPLC-UV; Non-steroidal anti-inflammatory drugs; Sol-gel technique; Metal-organic framework
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In this study, Cu-based metal-organic framework (MOF-199) was fabricated on stainless steel mesh through sol-gel procedure for extraction and quantification of NSAIDs from human plasma and water samples. By optimizing extraction conditions, linear calibration graphs and high correlation coefficients were achieved, with LODs ranging from 0.01 to 0.02 ng mL-1 for water samples and 0.03 to 0.1 ng mL-1 for plasma samples. The method showed good repeatability and relative recoveries between 93.6% to 99.6%.
In this research, the Cu-based metal-organic framework (MOF-199) was fabricated and coated on the stainless steel mesh as substrates through sol -gel procedure. Then the coated substrates were placed in a small column known as solid-phase extraction cartridge. The SPE based coated stainless steel mesh coupled with high-performance liquid chromatography-UV detector (HPLC-UV) was used for the fast extraction, and quantification of non-steroidal anti-inflammatory drugs (NSAIDs) from human plasma and water samples. To find optimum extraction conditions, the impacts of effective parameters on analytical performance like sample pH, sample volume, type, and volume of desorption solvent were optimized. At the optimized conditions, calibration graphs of analytes were linear in the concentration range of 0.03 -300 ng mL -1 for water samples, and 0.1 -200 ng mL -1 for plasma samples. The correlation coefficients were in the range of 0.9938 to 0.9989. Also, the limits of detection (LODs) were from 0.01 to 0.02 ng mL -1 for water samples and 0.03 to 0.1 ng mL -1 for plasma samples. The cartridge repeatability was studied at different values, and the relative standard deviations (RSDs%) were achieved between 3.5 and 5.1%. Consequently, this procedure was successfully used in the extraction and detection of NSAIDs in real water and plasma samples with relative recoveries ranged from 93.6 to 99.6%. (c) 2021 Elsevier B.V. All rights reserved.
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