Protein adsoprotein adsorption on core-shell particles: Comparison of CaptoTM Core 400 and 700 resins

Structural and functional characteristics of the two core-shell resins Capto (TM) Core 400 and 700, which are useful for the flow-through purification of bioparticles such as viruses, viral vectors, and vaccines, are compared using bovine serum albumin (BSA) and thyroglobulin (Tg) as models for small and large protein contaminants. Both resins are agarose-based and contain an adsorbing core surrounded by an inert shell. Although shell thicknesses are comparable (3.6 and 4.2 mu m for Capto Core 400 and 700, respectively), the two resins differ substantially in pore size (pore radii of 19 and 50 nm, respectively). Because of the smaller pores and higher surface area, the BSA binding capacity of Capto Core 400 is approximately double that of Capto Core 700. However, for the much larger Tg, the attainable capacity is substantially larger for Capto Core 700. Mass transfer in both resins is affected by diffusional resistances through the shell and within the adsorbing core. For BSA, core and shell effective pore diffusivities are about 0.25 x 10(-7) and 0.6 x 10(-7) cm(2)/s, respectively, for Capto Core 400, and about 1.6 x 10(-7) and 2.6 x 10(-7) cm(2)/s, respectively, for Capto Core 700. These values decrease dramatically for Tg to 0.022 x 10(-7) and 0.088 x 10(-7) cm(2)/s and to 0.13 x 10(-7) and 0.59 x 10(-7) cm(2)/s for Capto Core 400 and 700, respectively. Adsorbed Tg further hinders diffusion of BSA in both resins. Column measurements show that, despite the higher static capacity of Capto Core 400 for BSA, the dynamic binding capacity is greater for Capto Core 700 as a result of its faster kinetics. However, some of this advantage is lost if the feed is a mixture of BSA and Tg since, in this case, Tg binding leads to greater diffusional hindrance for BSA. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The structural and functional characteristics of two core-shell resins, Capto Core 400 and 700, were compared for the purification of bioparticles. While Capto Core 400 has higher BSA binding capacity, Capto Core 700 shows greater capacity for larger proteins like Tg. Mass transfer in both resins is influenced by diffusional resistances, with different effective pore diffusivities for small and large proteins.