Capturing Membrane Phase Separation by Dual Resolution Molecular Dynamics Simulations

Understanding the lateral organization in plasma membranes remains an open problem and is of great interest to many researchers. Model membranes consisting of coexisting domains are commonly used as simplified models of plasma membranes. The coarse-grained (CG) Martini force field has successfully captured spontaneous separation of ternary membranes into a liquid-disordered and a liquid-ordered domain. With all-atom (AA) models, however, phase separation is much harder to achieve due to the slow underlying dynamics. To remedy this problem, here, we apply the virtual site (VS) hybrid method on a ternary membrane composed of saturated lipids, unsaturated lipids, and cholesterol to investigate the phase separation. The VS scheme couples the two membrane leaflets at CG and AA resolution. We found that the rapid phase separation reached by the CG leaflet can accelerate and guide this process in the AA leaflet.

Automated summary

Research on lateral organization in plasma membranes remains a challenging issue, with simplified models often used. While coarse-grained models have shown success in capturing phase separation, achieving the same with all-atom models is more difficult. By applying the virtual site hybrid method, researchers were able to accelerate and guide phase separation in a ternary membrane.