An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons

Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (similar to 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63-3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect.

Automated summary

The study presents a novel non-lethal method to modify Drosophila CHCs profiles using high dose ozone gas exposure, resulting in a significant reduction in unsaturated hydrocarbons and subsequent regeneration within 108 hours. Desiccation resistance in D. suzukii was reduced one hour after ozone treatment but showed an increase compared to controls at 108 hours.