Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 25, Issue 14, Pages 6695-6708Publisher
WILEY
DOI: 10.1111/jcmm.16672
Keywords
cell adhesion; cytoskeleton; hierarchical micro; nano structure; mTORC2; osteogenic differentiation
Categories
Funding
- National Natural Science Foundation of China [81970958]
- Tianjin Research Innovation Project for Postgraduate Students [2019YJSS170]
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In this study, hierarchical micro/nano topography was achieved using selective laser melting combined with alkali heat treatment (SLM-AHT) to investigate osteogenesis mechanisms. The results demonstrated that SLM-AHT surface could enhance osteogenesis by upregulating cell adhesion-related molecules and promoting cytoskeleton reorganization.
Surface topography acts as an irreplaceable role in the long-term success of intraosseous implants. In this study, we prepared the hierarchical micro/nano topography using selective laser melting combined with alkali heat treatment (SLM-AHT) and explored the underlying mechanism of SLM-AHT surface-elicited osteogenesis. Our results show that cells cultured on SLM-AHT surface possess the largest number of mature FAs and exhibit a cytoskeleton reorganization compared with control groups. SLM-AHT surface could also significantly upregulate the expression of the cell adhesion-related molecule p-FAK, the osteogenic differentiation-related molecules RUNX2 and OCN as well as the mTORC2 signalling pathway key molecule Rictor. Notably, after the knocked-down of Rictor, there were no longer significant differences in the gene expression levels of the cell adhesion-related molecules and osteogenic differentiation-related molecules among the three titanium surfaces, and the cells on SLM-AHT surface failed to trigger cytoskeleton reorganization. In conclusion, the results suggest that mTORC2 can regulate the hierarchical micro/nano topography-mediated osteogenesis via cell adhesion and cytoskeletal reorganization.
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