4.5 Article

Immunoproteasome impairment via β5i/LMP7-deletion leads to sustained pancreatic injury from experimental pancreatitis

Journal

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 25, Issue 14, Pages 6786-6799

Publisher

WILEY
DOI: 10.1111/jcmm.16682

Keywords

cell death; cytokines; ER stress; pancreatitis; PSMB8

Funding

  1. Center of Excellence Mecklenburg-Vorpommern [ESF/14-BM-A55-0045/16]

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Acute pancreatitis is characterized by severe systemic inflammatory response and immunoproteasome plays a protective role by clearing damaged proteins and balancing ER stress responses in the pancreas.
Uncovering potential new targets involved in pancreatitis may permit the development of new therapies and improvement of patient's outcome. Acute pancreatitis is a primarily sterile disease characterized by a severe systemic inflammatory response associated with extensive necrosis and a mortality rate of up to 24%. Considering that one of the reported disease mechanisms comprises the endoplasmic reticulum (ER) stress response and that the immunoproteasome is a key regulator to prevent proteotoxic stress in an inflammatory context, we investigated its role in acute pancreatitis. In this study, we demonstrate that immunoproteasome deficiency by deletion of the beta 5i/LMP7-subunit leads to persistent pancreatic damage. Interestingly, immunoproteasome-deficient mice unveil increased activity of pancreatic enzymes in the acute disease phase as well as higher secretion of Interleukin-6 and transcript expression of the Interleukin IL-1 beta, IFN-beta cytokines and the CXCL-10 chemokine. Cell death was increased in immunoproteasome-deficient mice, which appears to be due to the increased accumulation of ubiquitin-protein conjugates and prolonged unfolded protein response. Accordingly, our findings suggest that the immunoproteasome plays a protective role in acute pancreatitis via its role in the clearance of damaged proteins and the balance of ER stress responses in pancreatic acini and in macrophages cytokine production.

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