Journal
JOURNAL OF CELL SCIENCE
Volume 134, Issue 13, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.257956
Keywords
Oocyte; Fertilization; Ca2+; Signaling; TRP channel
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Funding
- Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health (NIH) [HD051872, HD092499]
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By knocking out TRPV3 and Ca(V)3.2, we demonstrated their essential roles in completing Ca2+ store filling in mouse oocytes and eggs, as well as in initiating and maintaining periodic oscillations during mammalian fertilization.
Ca2+ influx during oocyte maturation and after sperm entry is necessary to fill the internal Ca2+ stores and for complete egg activation. We knocked out the transient receptor potential vanilloid member 3 (TRPV3) and the T-type channel, Ca(V)3.2, to determine their necessity for maintaining these functions in mammalian oocytes/eggs. Double-knockout (dKO) females were subfertile, their oocytes and eggs showed reduced internal Ca2+ stores, and, following sperm entry or Plcz (also known as Plcz1) cRNA injection, fewer dKO eggs displayed Ca2+ responses compared to wild-type eggs, which were also of lower frequency. These parameters were rescued and/or enhanced by removing extracellular Mg2+, suggesting that the residual Ca2+ influx could be mediated by the TRPM7 channel, consistent with the termination of divalent-cation oscillations in dKO eggs by a TRPM7 inhibitor. In total, we demonstrated that TRPV3 and Ca(V)3.2 mediate the complete filling of the Ca2+ stores in mouse oocytes and eggs. We also showed that they are required for initiating and maintaining regularly spaced-out oscillations, suggesting that Ca2+ influx through PM ion channels dictates the periodicity and persistence of Ca2+ oscillations during mammalian fertilization.
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