Journal
FOOD CHEMISTRY
Volume 192, Issue -, Pages 250-259Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.07.009
Keywords
DNA-binding activity; Feruloylhexaric acids; Flavonoids; Folium Citri Reticulatae; HPLC-DAD-MSn-EB-DNA-FLD; p-Coumaroylhexaric acids
Funding
- National Science and Technology Major Project: Safety Testing Technology of the New Drug of TCM and the Standard Research [2014ZX09304307-001-011]
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To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MSn-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MSn fragments and DNA-binding activity profile of various components in Folium Cirri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices. (C) 2015 Elsevier Ltd. All rights reserved.
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