Journal
JOURNAL OF BONE AND MINERAL METABOLISM
Volume 39, Issue 6, Pages 934-943Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00774-021-01240-3
Keywords
Osteoporosis; Calcium phosphate cement; Selenium; Oxidative stress; OPG; RANKL signaling pathway
Funding
- National Natural Science Foundation of China [82002322]
- Yijishan Hospital, Wannan Medical College [GF2019G04, PF2019005, GF2019T02, PF2019007]
- Young and Middle-aged Key Project of Wannan Medical College [WK2020ZF16]
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The study demonstrated that local administration of selenium with calcium phosphate cement enhanced the therapeutic effect in treating osteoporotic bone defects. The Se-CPC group showed the strongest effect on bone regeneration and mineralization compared to the CPC group and the OVX group. This effect may be achieved by inhibiting local oxidative stress and through the OPG/RANKL signaling pathway.
Objective The purpose is to observe whether local administration with selenium (Se) can enhance the efficacy of calcium phosphate cement (CPC) in the treatment of osteoporotic bone defects. Methods Thirty ovariectomized (OVX) rats with two defects were generated and randomly allocated into the following graft study groups: (1) OVX group (n = 10), (2) CPC group (n = 10); and (3) Se-CPC group (n = 10). Then, these selenium-modified calcium phosphate cement (Se-CPC) scaffolds were implanted into the femoral epiphysis bone defect model of OVX rats for 12 weeks. Micro-CT, history, western blot and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis were used to observe the therapeutic effect and to explore the possible mechanism. Result Micro-CT and histological analysis evaluation showed that the Se-CPC group presented the strongest effect on bone regeneration and bone mineralization when compared with the CPC group and the OVX group. Protein expressions showed that the oxidative stress protein expressions, such as SOD2 and GPX1 of the Se-CPC group, are significantly higher than those of the OVX group and the CPC group, while Se-CPC remarkably reduced the expression of CAT. RT-qPCR analysis showed that the Se-CPC group displayed more OPG than the OVX and CPC groups (p < 0.05), while Se-CPC exhibited less RANKL than the OVX and CPC groups (p < 0.05). Conclusion Our current study demonstrated that Se-CPC is a scheme for rapid repair of femoral condylar defects, and these effects may be achieved by inhibiting local oxidative stress and through OPG/RANKL signaling pathway.
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