Journal
FOOD CHEMISTRY
Volume 211, Issue -, Pages 631-636Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.05.109
Keywords
RAPD; SCAR; Ovine breeds; Milk adulteration; Serra da Estrela cheese; Quality control
Funding
- project Valor Queijo - FCT/MCTES (PIDDAC) [CENTRO-07-0202-FEDER-030372]
- Fundo Europeu de Desenvolvimento Regional (FEDER) through COMPETE - Programa Operacional Factores de Competitividade (POFC)
- Portuguese Foundation for Science and Technology (FCT) [DID/BID/04469/2013, POCI-01-0145FEDER-006684, RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462)]
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Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has a high commercial value. However, the adulteration of production with cheaper/lower-quality milks from non-autochthones ovine breeds compromises the quality of the final product and undervalues the original PDO cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of adulterant breeds in milk mixtures used for fraudulent production of this cheese. Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods. The RAPD-SCAR technique is here described, for the first time, to be potentially useful for detection of milk origin in dairy products. In this sense, our findings will play an important role on the valorization of Serra da Estrela cheese, as well as on other high-quality dairy products prone to adulteration, contributing to the further development of the dairy industry. (C) 2016 Elsevier Ltd. All rights reserved.
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