Selection of methanotrophic platform for methanol production using methane and biogas

To develop biotechnological process for methane to methanol conversion, selection of a suitable methanotrophic platform is an important aspect. Systematic approach based on literature and public databases was developed to select representative methanotrophs Methylotuvimicrobium alcaliphilum, Methylomonas methanica, Methylosinus trichosporium and Methylocella silvestris. Selected methanotrophs were further investigated for methanol tolerance and methanol production on pure methane as well as biogas along with key enzyme activities involved in methane utilization. Among selected methanotrophs M. alcaliphilum showed maximum methanol tolerance of 6% v/v along with maximum methanol production of 307.90 mg/L and 247.37 mg/L on pure methane and biogas respectively. Activity of methane monooxygenase and formate dehydrogenase enzymes in M. alcaliphilum was significantly higher up to 98.40 nmol/min/ mg cells and 0.87 U/mg protein, respectively. Biotransformation trials in 14 L fermentor resulted in increased methanol production up to 418 and 331.20 mg/L, with yield coefficient 0.83 and 0.71 mg methanol/mg of pure methane and biogas respectively. The systematic selection resulted in haloalkaliphilic strain M. alcaliphilum as one of the potential methanotroph for bio-methanol production. (c) 2021, The Society for Biotechnology, Japan. All rights reserved.

Automated summary

The study focused on selecting methanotrophs and investigating their methanol tolerance, production, and enzyme activities. M. alcaliphilum showed promising results for bio-methanol production, with high methanol tolerance and production levels. This systematic selection process identified M. alcaliphilum as a potential methanotroph for producing bio-methanol.