Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 297, Issue 5, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jbc.2021.101234
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Funding
- National Natural Science Foundation of China [31970468, 32030103]
- Natural Science Foundation of Chongqing, China [cstc2020jcyj-cxttX0001]
- Venture and Innovation Support Program for Chongqing Overseas Returnees [cx2020100]
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The study presents the crystal structures of JHAMT in various complexes, providing insights into the binding patterns, catalytic mechanism, and substrate recognition of this enzyme. The findings lay the foundation for understanding the mechanism of JH biosynthesis and offer a rational framework for the development of specific JHAMT inhibitors as insect growth regulators or insecticides.
Juvenile hormone (JH) acid methyltransferase (JHAMT) is a rate-limiting enzyme that converts JH acids or inactive precursors of JHs to active JHs at the final step of JH biosynthesis in insects and thus presents an excellent target for the development of insect growth regulators or insecticides. However, the three-dimensional properties and catalytic mechanism of this enzyme are not known. Herein, we report the crystal structure of the JHAMT apoenzyme, the three-dimensional holoprotein in binary complex with its cofactor S-adenosyl-L-homocysteine, and the ternary complex with S-adenosyl-L-homocysteine and its substrate methyl farnesoate. These structures reveal the ultrafine definition of the binding patterns for JHAMT with its substrate/cofactor. Comparative structural analyses led to novel findings concerning the structural specificity of the progressive conformational changes required for binding interactions that are induced in the presence of cofactor and substrate. Importantly, structural and biochemical analyses enabled identification of one strictly conserved catalytic Gln/His pair within JHAMTs required for catalysis and further provide a molecular basis for substrate recognition and the catalytic mechanism of JHAMTs. These findings lay the foundation for the mechanistic understanding of JH biosynthesis by JHAMTs and provide a rational framework for the discovery and development of specific JHAMT inhibitors as insect growth regulators or insecticides.
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