4.6 Article

Sequence variation in the β7-β8 loop of bacterial class A sortase enzymes alters substrate selectivity

Related references

Note: Only part of the references are listed.
Review Biochemistry & Molecular Biology

Engineered Sortases in Peptide and Protein Chemistry

Christian Freund et al.

Summary: The transpeptidase sortase A of Staphylococcus aureus is utilized in protein chemistry for sortase-mediated ligation. The moderate catalytic efficiency and specificity of Sa-SrtA have led to the development of new biocatalysts through screening of variants and directed protein evolution. This has resulted in sortases with enhanced catalytic activity and recognition of new sorting motifs.

CHEMBIOCHEM (2021)

Article Biochemistry & Molecular Biology

Laboratory evolution of a sortase enzyme that modifies amyloid-β protein

Christopher J. Podracky et al.

Summary: This study described the laboratory evolution of the bacterial transpeptidase sortase A to recognize specific sequences in the endogenous amyloid-beta (Aβ) protein, enabling site-specific protein modification without genetic manipulation. The evolved sortase showed a significantly changed substrate preference and was able to label endogenous Aβ in human cerebrospinal fluid with high sensitivity, demonstrating potential future applications in sortase-mediated labeling of Aβ peptides.

NATURE CHEMICAL BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Functional analysis of Clostridium difficile sortase B reveals key residues for catalytic activity and substrate specificity

Chia-Yu Kang et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2020)

Article Biochemical Research Methods

Designed Streptococcus pyogenes Sortase A Accepts Branched Amines as Nucleophiles in Sortagging

Zhi Zou et al.

BIOCONJUGATE CHEMISTRY (2020)

Article Biochemistry & Molecular Biology

Engineering the specificity ofStreptococcus pyogenessortase A by loop grafting

Magdalena Wojcik et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2020)

Article Biochemistry & Molecular Biology

Modular Polymer Antigens To Optimize Immunity

Nitasha R. Bennett et al.

BIOMACROMOLECULES (2019)

Article Biochemistry & Molecular Biology

MotifAnalyzer-PDZ: A computational program to investigate the evolution of PDZ-binding target specificity

Jordan Valgardson et al.

PROTEIN SCIENCE (2019)

Article Biochemistry & Molecular Biology

UniProt: a worldwide hub of protein knowledge

Alex Bateman et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Biochemical Research Methods

MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization

Kazutaka Katoh et al.

BRIEFINGS IN BIOINFORMATICS (2019)

Article Biochemistry & Molecular Biology

Expanding the Scope of Sortase-Mediated Ligations by Using Sortase Homologues

Keyvan D. Nikghalb et al.

CHEMBIOCHEM (2018)

Article Biochemistry & Molecular Biology

Database Resources of the National Center for Biotechnology

Richa Agarwala et al.

NUCLEIC ACIDS RESEARCH (2017)

Article Biochemistry & Molecular Biology

Engineering sortase A by screening a second-generation library using phage display

Lena Schmohl et al.

JOURNAL OF PEPTIDE SCIENCE (2017)

Article Biochemistry & Molecular Biology

Identification of sortase substrates by specificity profiling

Lena Schmohl et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2017)

Article Biochemistry & Molecular Biology

Recent advances in sortase-catalyzed ligation methodology

John M. Antos et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2016)

Article Biochemistry & Molecular Biology

ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules

Haim Ashkenazy et al.

NUCLEIC ACIDS RESEARCH (2016)

Article Biophysics

Site-specific immobilization of protein layers on gold surfaces via orthogonal sortases

Maryam Raeeszadeh-Sarmazdeh et al.

COLLOIDS AND SURFACES B-BIOINTERFACES (2015)

Review Biochemistry & Molecular Biology

Molecular features of the sortase enzyme family

William J. Bradshaw et al.

FEBS JOURNAL (2015)

Article Multidisciplinary Sciences

Reprogramming the specificity of sortase enzymes

Brent M. Dorr et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Biochemical Research Methods

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Carla P. Guimaraes et al.

NATURE PROTOCOLS (2013)

Article Biochemical Research Methods

Orthogonal Labeling of M13 Minor Capsid Proteins with DNA to Self-Assemble End-to-End Multiphage Structures

Gaelen T. Hess et al.

ACS SYNTHETIC BIOLOGY (2013)

Article Chemistry, Multidisciplinary

Directed Evolution of Sortase A Mutants with Altered Substrate Selectivity Profiles

Kirill Piotukh et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

Review Biochemistry & Molecular Biology

Sortase enzymes in Gram-positive bacteria

Thomas Spirig et al.

MOLECULAR MICROBIOLOGY (2011)

Article Multidisciplinary Sciences

A general strategy for the evolution of bond-forming enzymes using yeast display

Irwin Chen et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Mathematical & Computational Biology

UniProt Knowledgebase: a hub of integrated protein data

Michele Magrane

DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION (2011)

Review Biochemistry & Molecular Biology

Sortase Transpeptidases: Insights Into Mechanism, Substrate Specificity, and Inhibition

Kathleen W. Clancy et al.

BIOPOLYMERS (2010)

Article Biochemistry & Molecular Biology

Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations

Maximilian W. Popp et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Biochemistry & Molecular Biology

Crystal Structure of Streptococcus pyogenes Sortase A IMPLICATIONS FOR SORTASE MECHANISM

Paul R. Race et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Chemistry, Multidisciplinary

Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity

John M. Antos et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Biochemical Research Methods

Protein structure homology modeling using SWISS-MODEL workspace

Lorenza Bordoli et al.

NATURE PROTOCOLS (2009)

Article Chemistry, Organic

Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation

Stephan Pritz et al.

JOURNAL OF ORGANIC CHEMISTRY (2007)

Review Microbiology

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria

LA Marraffini et al.

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS (2006)

Article Biochemical Research Methods

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling

K Arnold et al.

BIOINFORMATICS (2006)

Article Biochemistry & Molecular Biology

ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures

M Landau et al.

NUCLEIC ACIDS RESEARCH (2005)

Article Biochemistry & Molecular Biology

Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA

RG Kruger et al.

BIOCHEMISTRY (2004)

Article Biochemical Research Methods

The Jalview Java alignment editor

M Clamp et al.

BIOINFORMATICS (2004)

Article Biochemistry & Molecular Biology

T-Coffee: A novel method for fast and accurate multiple sequence alignment

C Notredame et al.

JOURNAL OF MOLECULAR BIOLOGY (2000)