Validated UV Spectrophotometric Methods for the Determination of Anticancer Drug Erlotinib Hydrochloride in Bulk and Tablet Formulations

Two different simple, accurate, and precise UV spectrophotometric methods have been developed for the estimation of erlotinib hydrochloride in bulk and tablet forms by the zero-order (method I) and the zero-order AUC (method II). The drug was dissolved in a phosphate buffer solution (pH 7.4), and at 228.20 nm the zero-order (method I) and at 224.20-230.20 nm the zero-order AUC (method II) were developed. For both methods, erlotinib has linearity in the concentration range 10-70 mu g/mL, with a correlation coefficient R-2 > 0.99. Both methods showed good reproducibility and recovery with %RSD less than 2 and an accuracy of 98.54-101.39 and 98.08-99.83%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.16, 3.51, and 21.41, 64.90 mu g/mL, respectively, for both methods.

Automated summary

Two different UV spectrophotometric methods were developed for the estimation of erlotinib hydrochloride, showing good linearity, reproducibility, and accuracy in the concentration range of 10-70 μg/mL. The limit of detection and quantification were found to be 1.16, 3.51, and 21.41, 64.90 μg/mL, respectively, for both methods.