Journal
FOOD CHEMISTRY
Volume 211, Issue -, Pages 494-501Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.05.079
Keywords
Partial least-squares (PLS); Multivariate curve resolution alternating least squares (MCR-ALS); Terahertz time domain spectroscopy (THz-TDS); Amino acid
Funding
- National Instrumentation Program [2012YQ140005]
- National Science Foundation of China [21275101]
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Terahertz time domain spectroscopy (THz-TDS) combined with chemometrics has been utilized for the qualitative and quantitative analysis of binary mixtures of L-glutamic acid and L-glutamine which have similar chemical structures and properties. The binary mixtures of amino acids were prepared with yellow foxtail millet matrix, substituted for polyethylene (PE) as previously reported. After proper pretreatment of absorption spectra, quantitative analysis was achieved by partial least squares (PLS) and interval partial least squares (iPLS) regressions. The performance of models was evaluated based on the root mean square error of prediction (RMSEP) and correlation coefficient (R-2) of cross-validations with bootstrapped Latin partitions as criterion. The iPLS yielded better results with low RMSEP (0.39 +/- 0.02%, 0.39 +/- 0.02%), and higher R-2 values (0.9904, 0.9906) for glutamine and glutamic acid comparing to the conventional PLS models. Multivariate curve resolution alternating least squares (MCR-ALS) was successfully applied for resolution of pure THz spectra and concentration profiles of two amino acids components from mixtures. (C) 2016 Published by Elsevier Ltd.
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