Journal
FOOD CHEMISTRY
Volume 204, Issue -, Pages 444-452Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.02.159
Keywords
Dual-emission ratiometric fluorescence probe; Silica nanoparticles; FITC; Quantum dots; Glucose; Beverage
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A novel dual emission ratiometric fluorescence probe for determination of glucose has been developed. The reference dye fluorescence isothiocyanate (FITC) has been encapsulated in the silica nanoparticles and then the red emission CdTe QDs were grafted on the surface of the silica particles to obtain the fluorescence probe. With glucose and dopamine as substrates, the glucose level was proportional to the fluorescence ratio change of above probe caused by dopamine oxidation, which was produced via bienzyme catalysis (glucose oxidase and horseradish peroxidase). The established approach was sensitive and selective, and has been applied to determine the glucose in beverage, urine and serum samples. The average recoveries of the glucose at various spiking levels ranged from 95.5% to 108.9% with relative standard deviations from 1.5% to 4.3%. The results provided a clue to develop sensors for rapid determination of the target analytes from complex matrices. (C) 2016 Elsevier Ltd. All rights reserved.
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