Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 32, Pages 9313-9325Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c03850
Keywords
purple sweet potato; polysaccharide; immunomodulatory activity; toll-like receptor 2; toll-like receptor 4; pathway; molecular docking; biolayer interferometry
Funding
- Natural Science Foundation of Heilongjiang Province [LH2020H071]
- Application Technology Research and Development Plan of Heilongjiang Province [GA20B301]
- Science and Technology Project of Heilongjiang Administration of Traditional Chinese Medicine [ZHY2020-175]
- Talent Project of Harbin University of Commerce [2020CX37, 2020CX38, 2020CX09]
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PSPP-1, derived from purple sweet potato, enhanced immune modulation on macrophage cells through TLR2- and TLR4-mediated pathways. It may serve as a potential immunomodulatory agent for promoting immune functions.
PSPP-1 was obtained from purple sweet potato, and the effects of PSPP-1 on the immune modulation on macrophage cells were investigated for the first time. PSPP-1 promoted RAW264.7 proliferation and increased the total cell percentage in DNA synthesis and mitosis phases, and the cell morphology changed in volume and appearance. Additionally, the RAW264.7 immune functions of phagocytic activity and nitric oxide, reactive oxygen species, and cytokine production were improved by PSPP-1. The western blot experiment showed that PSPP-1 could activate toll-like receptor 2 and toll-like receptor 4-mediated pathways, and the expressions of proteins in MyD88-dependent, mitogen-activated protein kinase (MAPK)-signaling, NF-kappa B-signaling, AP-1 signaling, and TRIF-dependent pathways were improved markedly. Molecular docking and Biolayer Interferometry study further indicated that PSPP-1 could recognize and bind TLR2 and TLR4 by targeting the binding sites with a strong affinity. It suggested that PSPP-1 could enhance immunity via TLR2- and TLR4-mediated pathways, and it could be explored as an immunomodulatory agent.
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