Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 28, Issue 2, Pages 288-298Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2016.1263987
Keywords
Monoclonal antibody; norfloxacin; icELISA; immunochromatographic strip
Funding
- National Natural Science Foundation of China [21522102, 21503095, 21471068, 31400848, 21471128]
- MOST [2016YFD0401101]
- Natural Science Foundation of Jiangsu Province [BK20150145, BX20151038, BK20140003, BE2014672]
- MOF [BK20150145, BX20151038, BK20140003, BE2014672]
- MOE [BK20150145, BX20151038, BK20140003, BE2014672]
Ask authors/readers for more resources
We developed a monoclonal antibody (mAb) to detect norfloxacin (NOR) using a novel hapten by introducing an amino group via amido linkage into the piperazine ring of NOR. The sensitivity of an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on this mAb showed an improved IC50 value from 1.18 to 0.12ng/mL and the limit of detection from 0.37 to 0.05ng/mL by using a heterologous coating (pazufloxacin coupled to ovalbumin). The recovery rate for NOR in milk ranged from 77.3% to 117.8%. Cross-reactivity (CR) experiments showed good CR with nine common fluoroquinolone analogs: pefloxacin, lomfloxacin, ciprofloxacin, enrofloxacin, dafloxacin, sparfloxacin, ofloxacin, marbofloxacin and flumequine. Furthermore, based on this antigen-antibody combination, an immunochromatographic strip with cut-off value of 1ng/mL was developed. Given their good sensitivity and CR, the developed icELISA and immunochromatographic strip could be useful for detection of NOR and its analogs in milk.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available