Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 27, Issue 5, Pages 689-699Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2016.1148668
Keywords
Pentachlorophenol; ELISA; immunochromatographic strip
Funding
- Key Programs from MOST [2013AA065501, 2012BAK08B01]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK20140003, BK20150145, BK20150138, BE2013613, BE2013611]
Ask authors/readers for more resources
A sensitive monoclonal antibody for targeting pentachlorophenol was prepared with an immunization procedure and cell fusion. The antibody was characterized with an indirect competitive enzyme-linked immunosorbent assay (ELISA), which had an IC50 of 0.2ng/ml for pentachlorophenol under optimized conditions. The cross-reactivity of the ELISA to related compounds was very low. The recovery of pentachlorophenol was 99.2-105% in water samples and 89-110.9% in fish samples. A simple, rapid, and sensitive immunochromatographic strip was produced based on the monoclonal antibody, with a qualitative visual limit of detection of 2ng/ml in water samples and 10ng/ml in fish samples, indicating its potential utility in monitoring pentachlorophenol in the field.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available