Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 27, Issue 6, Pages 772-782Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2016.1160367
Keywords
Kunitz trypsin inhibitor; sandwich ELISA; immunochromatographic strip; soybean; monoclonal antibody
Funding
- MOST [2014BAD04B10, 2012BAD29B05]
- Natural Science Foundation of Jiangsu Province [BE2013613]
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Kunitz trypsin inhibitor (KTI) is a predominant anti-nutritional factor in soybean. The objective of this study was to develop a sandwich enzyme-linked immunosorbent assay and immunochromato graphic strip (ICS) method for the semi-quantification of KTI in soybean. The limit of detection was 0.1481ng/ml, and the linear dynamic range was 0.2-20ng/ml. KTI recovery was 98.40-110.66% from KTI-spiked milk samples, with coefficients of variation of 4.41-10.62%. Based on ICS results, KTI had cutoff values of 5ng/ml and 2.5ng/ml in 0.05% Tween-added phosphate-buffered saline and milk, respectively. ICS has potential applications for the fast detection of KTI.
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