Journal
FOOD ANALYTICAL METHODS
Volume 9, Issue 8, Pages 2335-2341Publisher
SPRINGER
DOI: 10.1007/s12161-016-0416-7
Keywords
Carotenoids; Red mamey fruit; Multidimensional chromatography; LCxUHPLC-PDA-APCI/MS
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Funding
- Shimadzu Corporation
- Sigma-Aldrich/Supelco Corporation
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One-dimensional liquid chromatography is widely applied to the analytical separation of various phytochemicals, including carotenoids; however, sometimes it does not provide sufficient resolution power for the analyte separation in very complex real-world samples. Multidimensional chromatography is a technique that may provide the required analytical separation power to tackle such a task. The aim of this work was to apply comprehensive multidimensional two-dimensional liquid chromatography, coupled to photodiode array and atmospheric pressure chemical ionization mass spectrometry detection, to the free carotenoid separation in red mamey (Pouteria sapote) fruit for the first time, using ultra high pressure liquid chromatography conditions in the second dimension separation. A comparison between one- and two-dimensional separation of the same matrix is shown, and a total of 23 different carotenoids belonging to 17 different carotenoid chemical classes were separated in the double orthogonal separation and identification system; moreover, a hypothesis for the possible detection of a new carotenoid is also reported for the first time.
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