Flavimobilis rhizosphaerae sp. nov., isolated from rhizosphere soil of Spartina alterniflora

A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621(T), was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach. GY 10621(T) was positive for catalase and oxidase. Growth occurred at 4-42 degrees C (optimum 30-37 degrees C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%). The main menaquinones were MK-9 (H-4) (92.2%) and MK-10 (7.8%). The major cellular fatty acids were anteiso-C-15:0 and C-14:0. The peptidoglycan was the type A4 alpha (L-Lys-Ser-d-Glu). The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids. The DNA G+C content of the type strain was 71.7 mol%. On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621(T) was Flavimobilis soli KCTC 13155(T) (97.16 %), followed by Sanguibacter suarezii NBRC 16159(T) (96.39 %). The calculated results indicated that compared with GY 10621(T), the average nucleotide identity (ANI) values of three strains closely related to GY 10621(T) (the two aforementioned type strains and 'S. massiliensis' Marseille-P3815) were 74.18-94.97%, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6%. The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621(T) should be assigned to the genus Flavimobilis. On the basis of evidence from polyphasic studies, GY 10621(T) should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp. nov. is proposed. The type strain is GY 10621(T) (=CGMCC 1.17411(T)=KCTC 49515(T)).

Automated summary

A novel Gram-stain positive bacterium GY 10621(T) was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and assigned to the genus Flavimobilis based on polyphasic studies. The strain showed catalase and oxidase activity, and could grow in a wide range of temperature, pH, and salt concentrations.