Gulosibacter sediminis sp. nov., isolated from Indian Ocean marine sediment

A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148(T), was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 degrees C. pH 8.0 and in the presence of 1-3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M12148(T) belongs to the genus Gulosibacter, with the highest sequence similarity to Gulosibacter faecalis NBRC 15706(T) (96.12%). The cell-wall sugars of strain YIM M12148(T) were rhamnose, ribose, glucose and mannose. The predominant isoprenoid quinones were MK-8 and MK-9. The polar lipids consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown lipid. Major fatty acids (>5% of the total) of the novel isolate were anteiso-C-15:0, iso-C-10:0, iso-C-13:0 and anteiso-C-13:0. The genomic DNA G+C content of strain YIM M12148(T) was 67.15 mol%. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M12148(T) represents a novel species of the genus Gulosibacter, for which the name Gulosibacter sediminis sp. nov. is proposed. The type strain is YIM M12148(T) (=KCTC 29660(T)=DSM 29154(T)).

Automated summary

The novel bacterium strain YIM M12148(T) isolated from a marine sediment sample from the Indian Ocean belongs to the genus Gulosibacter. It grows optimally at 28 degrees C, pH 8.0, and in the presence of 1-3% NaCl. Phylogenetic analysis shows high similarity to Gulosibacter faecalis NBRC 15706(T).