Curvivirga aplysinae gen. nov., sp. nov., a marine bacterium isolated from the sea sponge Aplysina fistularis

Aplysina fistularis, collected off the west coast of San Salvador, The Bahamas. Cells were curved- to- spiral rods with single, bipolar (amphitrichous) flagella, oxidase- and catalase- positive, non- nitrate- reducing and required salt for growth. RKSG073T grew optimally at 30-37 degrees C, pH 6-7, and with 2-3 % (w/v) NaCl. The predominant fatty acids of RKSG073T were summed feature 8 (C18:1 omega 6c and/or C18:1 omega 7c) and C16:0. Major isoprenoid quinones were identified as Q-10 and Q-9. Phylogenetic analyses of nearly complete 16S rRNA genes and genome sequences positioned strain RKSG073T in a clade with its closest relative Aestuariispira insulae AH-MY2T (92.1 % 16S rRNA gene sequence similarity), which subsequently clustered with Hwanghaeella grinnelliae Gri0909T, Marivibrio halodurans ZC80T and type species of the genera Kiloniella, Thalassospira and Terasakiella. The DNA G+C content calculated from the genome of RKSG073T was 42.2 mol%. On the basis of phylogenetic distinctiveness and polyphasic analysis, here we propose that RKSG073T (culture deposit numbers: ATCC collection = TSD-74T, BCCM collection = LMG 29869T) represents the type strain of a novel genus and species within the family Kiloniellaceae, order Rhodospirillales and class Alphaproteobacteria, for which the name Curvivirga aplysinae gen. nov., sp. nov. is proposed.

Automated summary

The bacterium RKSG073T isolated from Aplysina fistularis off the coast of San Salvador showed optimal growth conditions at 30-37 degrees C, pH 6-7, and with 2-3 % NaCl. Phylogenetic analysis placed it in a clade with its closest relative Aestuariispira insulae AH-MY2T. Based on its distinctiveness, a novel genus and species Curvivirga aplysinae gen. nov., sp. nov. is proposed for RKSG073T.