Peptoniphilus faecalis sp. nov., isolated from swine faeces

An obligately anaerobic, Gram-positive, non-motile, coccus-shaped bacterial strain designated AGMB00490(T) was isolated from swine faeces. 16S rRNA gene sequence-based phylogenetic analysis indicated that the isolate belongs to the genus Peptoniphilus and that the most closely related species is Peptoniphilus gorbachii WAL 10418(T) (=KCTC 5947(T), 97.22% 16S rRNA gene sequence similarity). Whole genome sequence analysis determined that the DNA G+C content of strain AGMB00490(T) was 31.2 mol% and moreover that the genome size and numbers of tRNA and rRNA genes were 2129517 bp, 34 and 10, respectively. Strain AGMB00490(T) was negative for oxidase and urease; positive for catalase, indole production, arginine arylamidase, leucine arylamidase, tyrosine arylamidase and histidine arylamidase; and weakly positive for phenylalanine arylamidase and glycine arylamidase. The major cellular fatty acids (>10 %) of the isolate were determined to be C-16:0 and C-18:1 omega 9c. Strain AGMB00490(T) produced acetic acid as a major end product of metabolism. Accordingly, phylogenetic, physiologic and chemotaxonomic analyses revealed that strain AGMB00490(T) represents a novel species for which the name Peptoniphilus faecalis sp. nov. is proposed. The type strain is AGMB00490(T) (=KCTC 15944(T)=NBRC 114159(T)).

Automated summary

AGMB00490(T) strain, isolated from swine faeces, belongs to the genus Peptoniphilus based on 16S rRNA gene sequence analysis. A new species, Peptoniphilus faecalis sp. nov., was proposed for this strain, which produces acetic acid as a major end product of metabolism.